The Biden administration has been reinforcing the hard line on exports to Huawei, denying licenses to sell chips to Huawei for use in or with 5g devices
The use of HarmonyOS means the company will no longer be wholly reliant on Android.
Huawei is billing HarmonyOS as an 'Internet-of-Things' platform, aimed at operating on and connecting other devices such as laptops, smartwatches, cars and appliances.
It became one of the world's three leading mobile phone manufacturers along with Samsung and Apple - briefly occupying the number one spot last year - driven by Chinese demand and sales in emerging markets.
Revenue rose to 76.88 billion yuan ($12 billion) in the quarter ended March 31, from 49.70 billion yuan a year earlier. Analysts expected revenue of 74.5 billion yen, according to Refinitiv data.
Adjusted net profit rose to 6.1 billion yuan, versus market estimates of 3.97 billion yuan.